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KMID : 1007520080170061372
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Food Science and Biotechnology
2008 Volume.17 No. 6 p.1372 ~ p.1375
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Heterologous Gene Expression of aprE2 Encoding a 29kDa Fibrinolytic Enzyme from Bacillus subtilis in Bacillus licheniformis ATCC 10716
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Kwon Gun-Hee
Jeong Woo-Ju
Lee Ae-Ran
Park Jae-Yong
Cha Jae-Ho
Song Young-Sun
Kim Jeong-Hwan
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Abstract
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The aprE2 gene from Bacillus subtilis CH3-5 was expressed in Bacillus licheniformis ATCC 10716 using aBacillus-Escherichai coli shuttle vector, pHY300PLK. The fibrinolytic activity of transformant (TF) increased significantlycompared to B. licheniformis 10716 control cell. During the 100hr incubation in Luria-Bertaini broth at 37oC, fibrinolyticactivity of B. licheniformis TF increased rapidly at the late growth stage, after 52hr of incubation, which was confirmed byzymography using a fibrin gel. pHY3-5 was stably maintained in B. licheniformis without tetracycline (Tc) in the media,60.9% of cells still maintained pHY3-5 after 100 hr of cultivation.
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KEYWORD
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Bacillus licheniformis, fibrinolytic enzyme, gene expression, transformation, Bacillus subtilis
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